anti rat igg f ab 2 apc cy7 Search Results


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Bio-Techne corporation goat anti-rat f(ab)2 igg (h+l) apc-conjugated antibody
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Jackson Immuno goat anti mouse igg gold
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Jackson Immuno fluorescein isothiocyanate fitc conjugated donkey
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
Fluorescein Isothiocyanate Fitc Conjugated Donkey, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc anti rabbit igg f ab 2
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
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Jackson Immuno anti goat igg fitc
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
Anti Goat Igg Fitc, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno goat anti human horseradish peroxidase labeled
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
Goat Anti Human Horseradish Peroxidase Labeled, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno anti human igg
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
Anti Human Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno dylight 488 goat anti rabbit
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
Dylight 488 Goat Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno goat anti mouse horseradish peroxidase hrp
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
Goat Anti Mouse Horseradish Peroxidase Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher fitc conjugated goat anti mouse igg f ab 2
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
Fitc Conjugated Goat Anti Mouse Igg F Ab 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abcam goat anti mouse igg f ab 2 fragment hrp
Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a <t>FITC-conjugated</t> second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.
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Image Search Results


Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a FITC-conjugated second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.

Journal: European journal of biochemistry

Article Title: N-terminal and C-terminal plasma membrane anchoring modulate differently agonist-induced activation of cytosolic phospholipase A2.

doi: 10.1046/j.1432-1327.1999.00797.x

Figure Lengend Snippet: Fig. 2. Subcellular distribution of wild-type cPLA2, myc-cPLA2, Lck-cPLA2 and cPLA2-Ras in CHO-2B cells. Parental CHO-2B cells (A) and cells transiently transfected with either wild-type cPLA2 (B), myc-cPLA2 (C), Lck-cPLA2 (D) or cPLA2-Ras (E) constructs were stained with a polyclonal anti-cPLA2 and a FITC-conjugated second antibody (green). Nuclei were stained with propidium iodide (red). Immuno- fluorescent staining was visualized by confocal microscopy. Note the plasma membrane distribution of Lck-cPLA2 (D) and cPLA2-Ras (E) as opposed to the punctate cytoplasmic distribution of wild-type cPLA2 (B) and myc-cPLA2 (C). Immunofluorescence analysis was also performed on stable transfectants producing the various forms of cPLA2. The patterns of staining were similar to those of the transiently transfected cells. Bar, 10 mm.

Article Snippet: They were then incubated with fluorescein isothiocyanate (FITC)-conjugated donkey anti-(rabbit IgG) (diluted 1 : 100; Jackson ImmunoResearch Laboratories) for 1 h, washed and incubated for 10 min with 1 mg ́mL21 RNAse A.

Techniques: Transfection, Construct, Staining, Confocal Microscopy, Clinical Proteomics, Membrane, Immunofluorescence